AQP11 antibody (Extracellular)

Catalog No. ABIN7042934

Product Details anti-AQP11 Antibody

Target: AQP11 (Aquaporin 11 (AQP11))

Binding Specificity: AA 140-151, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP11 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 11 Channel

Specificity: 2nd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - 11,12 amino acid residues identical

Characteristics: Anti-Aquaporin 11 (extracellular) Antibody is directed against an extracellular epitope of rat Aquaporin 11. Anti-Aquaporin 11 (extracellular) Antibody (ABIN7042934, ABIN7045207 and ABIN7045208)) can be used in western blot analysis. It has been designed to recognize AQP11 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SLSLTKYHFDER, corresponding to amino acid residues 140-151 of rat AQP11

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: BLP-QP011

Blocking Peptide: BLP-QP011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AQP11

Target: AQP11 (Aquaporin 11 (AQP11))

Alternative Name: AQP11 (AQP11 Products)

Background: AQP11,The aquaporins (AQP), also known as the major intrinsic protein superfamily (MIP), are a family of integral membrane proteins. In mammals, 13 aquaporins were described (AQP0-AQP12). Aquaporin 11 (AQP11) is a newly described member of the aquaporins family. Aquaporin 11 (AQP11) has been identified with unusual pore-forming NPA (asparagine-proline-alanine) boxes. AQP11 presents a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1. There are two subfamilies: the aquaporins, which transport only water and the aquaglyceroporins, which transport glycerol, urea, and other small solutes in addition to water2.Aquaporins and aquaglyceroporins are ubiquitously expressed, including in the plasma membrane of renal epithelia, brain astrocytes, and red blood cells3. AQP11 is expressed in several tissues including testis, kidney, liver, and brain. In the brain, AQP11 is found in Purkinje cells, hippocampal neurons of CA1 and CA2, and cerebral cortical neurons4. In the kidney AQP11 has a central role in vesicular homeostasis as demonstrated in experiments using AQP11 knockout mice which display growth retardation and progressively die before weaning. The cause of death seems to be advanced renal failure5.

Alternative names: Aquaporin 11, AQP11

Gene ID: 286758

NCBI Accession: NM_173039

UniProt: Q8CHM1