AVPR1A antibody (Intracellular)

Catalog No. ABIN7042946

Product Details anti-AVPR1A Antibody

Target: AVPR1A (Arginine Vasopressin Receptor 1A (AVPR1A))

Binding Specificity: AA 378-390, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AVPR1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Vasopressin V1A Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical,human - 10,13

Characteristics: Anti-Vasopressin V1A Receptor (AVPR1A) Antibody is directed against an epitope of rat Arginine vasopressin receptor 1A. Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (ABIN7042946, ABIN7045335 and ABIN7045336)) can be used in western blot analysis. It has been designed to recognize V1aR from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KFAKDDSDSMSRR, corresponding to amino acid residues 378-390 of rat AVPR1A 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-VR010

Blocking Peptide: BLP-VR010

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AVPR1A

Target: AVPR1A (Arginine Vasopressin Receptor 1A (AVPR1A))

Alternative Name: AVPR1A (AVPR1A Products)

Background: V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor,Vasopressin (AVP), the antidiuretic hormone, is a cyclic nonapeptide involved in the homeostasis of body fluid, blood volume, vascular tone, and blood pressure. AVP also belongs to the family of vasoactive and mitogenic peptides involved in physiological and pathological cell growth and differentiation1.AVP exerts its actions through binding to specific V1A, V1B, and V2, membrane receptors coupled to distinct second messengers2. V1 AVP receptor has the typical features of a G-protein coupled transmembrane receptor with seven putative hydrophobic domains, connected by three extracellular and three intracellular loops3.V1 receptors activate phospholipases A2, C, and D, resulting in the production of inositol 1,4,5-trisphosphate (IPS) and 1,Z-diacylglycerol (DAG), the mobilization of intracellular Ca2+, the influx of extracellular Ca2+, the activation of protein kinase C, and protein phosphorylation4. V1A AVP receptors have been shown by radioligand binding techniques to be present in vascular smooth muscle cells, hepatocytes, blood platelets, lymphocytes and monocytes, type 2 pneumocytes, adrenal cortex, brain (hippocampus septum et amygdalae), reproductive organs, retinal epithelium, renal mesangial cells, and the A10, A7r5,3T3, and WRK-1 cell lines4. V1A AVP receptors mediate cell contraction and proliferation, platelet aggregation, coagulation factor release, and glycogenolysis. V1B AVP receptors are located in the anterior pituitary where they stimulate ACTH release1.

Alternative names: Vasopressin V1A Receptor (AVPR1A), V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor

Gene ID: 25107

NCBI Accession: NM_000706

UniProt: P30560

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Response to Water Deprivation, Regulation of Carbohydrate Metabolic Process