AVPR1B antibody (Intracellular)

Catalog No. ABIN7042947

Product Details anti-AVPR1B Antibody

Target: AVPR1B (Arginine Vasopressin Receptor 1B (AVPR1B))

Binding Specificity: AA 399-412, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AVPR1B antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Vasopressin V1B Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - 12,14 amino acid residues identical, human - not recommended for use with human sample

Characteristics: Anti-Vasopressin V1B Receptor (AVPR1B) Antibody is directed against an epitope of rat Arginine vasopressin receptor 1B. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (ABIN7042947, ABIN7045337 and ABIN7045338)) can be used in western blot analysis. It has been designed to recognize V1bR from rat and mouse samples. The antibody is not recommended for human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RAKPRPAGSLKDLE, corresponding to amino acid residues 399-412 of rat AVPR1B

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-VR011

Blocking Peptide: BLP-VR011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AVPR1B

Target: AVPR1B (Arginine Vasopressin Receptor 1B (AVPR1B))

Alternative Name: AVPR1B (AVPR1B Products)

Background: V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor,Vasopressin V1b receptor (also called V3R) is a G protein-coupled receptor (GPCR) of the Gq type, thus activating the phospholipase C signaling pathway upon activation. It is part of a family of GPCRs triggered by vasopressin that further include V1R, V2R, oxytocin receptors and purinergic receptors. While V1R and V3R are Gq GPCRs, V2R is coupled to the Gs protein and activates adenylate cyclase1,2.The V1b receptor has an intracellular C-terminal, seven transmembrane domains, and an extracellular N-tail, the transmembrane and extracellular domains are responsible for interacting with agonists and to signal for activation of the coupled Gq receptor1,2.The V1b receptor is mainly detected in the corticotrophs of the anterior pituitary gland in the brain where it regulates the release of adrenocorticotrophic hormone (ACTH) upon stimulation of vasopressin-corticotrophin-releasing hormone conjugates2.V1b KO mice show that the receptor is critical for the functionality of the hypothalamic-pituitary-adrenal axis, and demonstrate its importance in regulating glucose levels. Recent studies have also suggested that lactating rats treated with V1b antagonists, were less nurturing and similarly aggressive compared with untreated rats and that mice with reduced expression of V1b and V1a receptors have shown resistance to jet-lag1,3,4.

Alternative names: Vasopressin V1B Receptor (AVPR1B), V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor

Gene ID: 100909648

NCBI Accession: NM_000707

UniProt: P48974

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones