Arginine Vasopressin Receptor 1B (AVPR1B) (Cytoplasmic Domain) antibody

Details for Product No. ABIN1048334, Supplier: Log in to see
Antigen
  • LOC443050
  • AVPR1B
  • si:ch211-125a15.3
  • AVPR3
  • V3/V1b
  • VIBR
  • VPR3
  • arginine vasopressin receptor 1B
  • arginine vasopressin receptor 1Ab
  • AVPR1B
  • avpr1ab
  • Avpr1b
Alternatives
anti-Human Arginine Vasopressin Receptor 1B antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Cytoplasmic Domain
47
17
11
11
10
7
5
4
4
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Monkey
100
56
51
6
4
4
3
2
2
1
1
1
Host
Rabbit
114
40
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
10
7
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
114
86
36
30
13
7
4
2
2
1
1
1
1
Options
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Brand IHC-plus™
Immunogen Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human AVPR1B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Bat, Pig (88%).

Type of Immunogen: Synthetic peptide
Specificity Human AVPR1B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Bat, Pig (88%).
Purification Immunoaffinity purified
Antigen Arginine Vasopressin Receptor 1B (AVPR1B)
Alternative Name AVPR1B (AVPR1B Antibody Abstract)
Background Name/Gene ID: AVPR1B
Subfamily: Vasopressin/oxytocin
Family: GPCR

Synonyms: AVPR1B, AVPR3, V1bR, AVPR V1b, AVPR V3, V1b receptor, V1b vasopressin receptor, Vasopressin V1b receptor, Vasopressin V3 receptor, VPR3
Gene ID 553
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones
Application Notes Approved: IHC, IHC-P (2 - 10 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (Cytoplasmic Domain) antibody (ABIN1048334) Anti-AVPR1B antibody ABIN1048334 IHC staining of human pituitary, anterior. Immunohi...
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (Cytoplasmic Domain) antibody (ABIN1048334) Anti-AVPR1B antibody IHC of human pituitary, anterior. Immunohistochemistry of formal...
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