ATP2B3 antibody (Intracellular)

Catalog No. ABIN7042957

Product Details anti-ATP2B3 Antibody

Target: ATP2B3 (ATPase, Ca++ Transporting, Plasma Membrane 3 (ATP2B3))

Binding Specificity: AA 10-23, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to PMCA3 ATPase

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Rat,mouse - 13, human - 11

Characteristics: Anti-PMCA3 Antibody (ABIN7042957, ABIN7044144 and ABIN7044145)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EFHPKPQ(N)QREVPH, corresponding to amino acid residues 10-23 of rat PMCA3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CP003

Blocking Peptide: BLP-CP003

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP2B3

Target: ATP2B3 (ATPase, Ca++ Transporting, Plasma Membrane 3 (ATP2B3))

Alternative Name: ATP2B3 (ATP2B3 Products)

Background: Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1,PMCAs (plasma membrane calcium ATPases) are P-type ATPases, a separate sub-group of Ca2 + pumps responsible for maintain intracellular Ca2+ homeostasis. PMCAs reduce Ca2 + in the cytosol by direct ejection, by controlling the formation of inositol-1,4,5-trisphosphate (IP3), and by decreasing Ca2 + release from the endoplasmic reticulum Ca2+ pool1,2.Four PMAC proteins are expressed in mammals: PMCA1-4. Alternative splicing produces more than twenty variants that are dissimilar in their regulatory characteristics1,3. PMCA pumps have a C-terminus with a high affinity calmodulin-binding site that also acts as an auto-inhibitor. The C-terminal region also has phosphorylation sites as well as proteolytic sites for caspase-3 and calpain and several motifs that control trafficking of the pump1,2.PMCA3 is usually present in excitable cells, in the brain and in hair cells of the inner ear or skeletal muscle. The variant is quickly activated and stays active for a long period of time1.

Alternative names: PMCA3, Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1

Gene ID: 29599

NCBI Accession: NM_021949

UniProt: Q64568

Pathways: Ribonucleoside Biosynthetic Process