ATP2B4 antibody (AA 601-617)

Catalog No. ABIN7042958

Product Details anti-ATP2B4 Antibody

Target: ATP2B4 (ATPase, Ca++ Transporting, Plasma Membrane 4 (ATP2B4))

Binding Specificity: AA 601-617

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to PMCA4 ATPase

Specificity: 2nd intracellular loop

Cross-Reactivity (Details): This antibody is not recommended for human samples.

Predicted Reactivity: Mouse - 12,17 amino acid residues identical

Characteristics: Anti-PMCA4 Antibody is directed against an epitope of rat PMCA4. Anti-PMCA4 Antibody (ABIN7042958, ABIN7044146 and ABIN7044147)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA4 from rat and mouse samples. It is unlikely to recognize PMCA4 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CDRILNKEGGIVPFKTK, corresponding to amino acid residues 601-617 of rat PMCA4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CP004

Blocking Peptide: BLP-CP004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP2B4

Target: ATP2B4 (ATPase, Ca++ Transporting, Plasma Membrane 4 (ATP2B4))

Alternative Name: ATP2B4 (ATP2B4 Products)

Background: Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4,Plasma membrane calcium pumps (PMCA) are part of the P2 subfamily of P-type primary ion transport ATPases. PMCAs are found in all eukaryotic cells and serve as a major high-affinity transporter for Ca2+ in the plasma membrane.PMCA4, as other PMCA pumps, is predicted to have 10 membrane spanning domains with the -NH2 and -COOH termini both located on the cytosolic side of the membrane. Most of the protein is facing the cytosol and is comprised of three main parts - the intracellular loop between transmembrane segments 2 and 3, the large unit between membrane-spanning domains 4 and 5, and an extended "tail" following the last transmembrane domain.PMCA4 is regulated by various regulatory mechanisms including Ca2+-Calmodulin and acidic phospholipids. Calmodulin binds to a region in the -COOH terminal located about 40 residues downstream from the last transmembrane domain. In the absence of calmodulin, this region acts as an auto-inhibitory domain. Acidic phospholipids are strong activators of PMCA4. They reduce the Michaelis-Menten constant for Ca2+ of the enzyme to roughly 0.3 μM compared with 0.4-0.7 μM in the case of calmodulin activation. Activation by phospholipids makes the PMCA insensitive to calmodulin thus suggesting that their effect is not additive1.PMCA4 deficiency does not affect embryonic development in mice. PMCA4 knockout mice are indistinguishable from wild type mice in terms of their behavior, appearance and weight. PMCA4 is highly expressed in the sperm tail and deletion of PMCA4 causes severely impaired sperm motility in mice2.

Alternative names: PMCA4, Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4

Gene ID: 29600

NCBI Accession: NM_001001396

UniProt: Q64542

Pathways: Ribonucleoside Biosynthetic Process