BDKRB1 antibody (Cys250Ser-Mutant)

Catalog No. ABIN7042967

Product Details anti-BDKRB1 Antibody

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Binding Specificity: AA 243-257, Cys250Ser-Mutant

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDKRB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Might not recognize the human epitope.

Predicted Reactivity: Mouse - 13,15 amino acid residues identical

Characteristics: Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (ABIN7042967, ABIN7043940 and ABIN7043941)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize B1R from rat and mouse samples. The antibody may not recognize the receptor from human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEAS RTR*SG GPKGS K, corresponding to amino acid residues 243-257 of rat B1R with replacement of cysteine 250 (C250) with serine (*S)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-BR011

Blocking Peptide: BLP-BR011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for BDKRB1

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Alternative Name: BDKRB1 (BDKRB1 Products)

Background: B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1,Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.

Alternative names: B1 Bradykinin Receptor (BDKRB1), B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1

Gene ID: 81509

NCBI Accession: NM_000710

UniProt: P97583

Pathways: ACE Inhibitor Pathway