ADGRB1 antibody (Extracellular)

Catalog No. ABIN7042971

Product Details anti-ADGRB1 Antibody

Target: ADGRB1 (Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1))

Binding Specificity: AA 97-112, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADGRB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to BAI1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody will recognize the intact BAI1 receptor as well as the proteolytically processed N-terminal fragment. This fragment is also known as Vasculostatin (Vstat120).

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-BAI1 (extracellular) Antibody (ABIN7042971 and ABIN7043944)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize BAI1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GRVRTYQFDSFLESTR, corresponding to amino acid residues 97-112 of mouse BAI1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: LCI

Negative Control: BLP-BR021

Blocking Peptide: BLP-BR021

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADGRB1

Target: ADGRB1 (Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1))

Alternative Name: ADGRB1 (ADGRB1 Products)

Background: Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF,The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-terminal tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large1,2. Many domains are localized to the N-terminus, various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-12,3. The C-terminal tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins2,4.Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain2. Following autocleavage, the N-terminal fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects2,5. At the mRNA level, all BaIs are expressed in fetal and adult human brain2,6. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain2.These receptors are implicated in various diseases and disorders such as primary glioma, pulmonary adenocarcinomas, gastric and colorectal cancers2,6,7.

Alternative names: BAI1, Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF

Gene ID: 107831

NCBI Accession: NM_001702

UniProt: Q3UHD1