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CACNB1 antibody (Intracellular)

CACNB1 Reactivity: Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7042984
  • Target See all CACNB1 Antibodies
    CACNB1 (Calcium Channel, Voltage-Dependent, beta 1 Subunit (CACNB1))
    Binding Specificity
    • 9
    • 8
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 456-471, Intracellular
    Reactivity
    • 42
    • 22
    • 11
    • 7
    • 7
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Rat
    Host
    • 40
    • 3
    Rabbit
    Clonality
    • 42
    • 1
    Polyclonal
    Conjugate
    • 31
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CACNB1 antibody is un-conjugated
    Application
    • 36
    • 13
    • 9
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit
    Specificity
    Intracellular, adjacent to the C-terminus
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 14,16
    Characteristics
    Anti-CACNB1 Antibody is directed against an epitope of rat CaVβ1. Anti-CACNB1 Antibody (ABIN7042984, ABIN7044049 and ABIN7044050) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CaVβ1 from rat, mouse and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat CACNB1 

    Isotype
    IgG
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    Discover our top product CACNB1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:800

    Comment

    Negative Control: (ABIN7234923)

    Blocking Peptide: (ABIN7234923)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    0.2 mL double distilled water (DDW).
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    CACNB1 (Calcium Channel, Voltage-Dependent, beta 1 Subunit (CACNB1))
    Alternative Name
    CACNB1 (CACNB1 Products)
    Background
    CaVβ1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1,Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, CaV channels have traditionally been classified into L, T, N, P, Q, and R types2. L-type Ca2+ channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the subunits α2δ, γ, and β3. CaVβ subunits play critical roles in membrane trafficking of the channel complex and regulation of voltage-dependent gating. The CaVβ subunit binds to the endoplasmic reticulum (ER) retention signal in the I-II loop of the α1-subunit, which allows channels to traffic to the surface membrane4. Furthermore, CaVβ subunits not only allow for membrane trafficking of the channel complex, they also can play a role in determining the subcellular localization of channels on the surface membrane5. There are four distinct CaVβ subunits CaVβ1, CaVβ2, CaVβ3 and CaVβ46.Three splice variants exist for the β1 subunit: β1a, β1b and β1c. β1a is known to be expressed in skeletal muscle and brain, but not in smooth muscle or heart. β1a appears to be important for the functional expression of the α1 subunit in skeletal muscle7. β1b was identified by cloning in rat brain, heart and hippocampus, and differs from β1a by having a deletion of ~50 amino acids at residue 209, and having a 120-residue C-terminal elongation. β1c was cloned from human heart and hippocampus and has the same deletion as β1b, but lacks the C-terminal extension.

    Alternative names: CACNB1, CaVbeta1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1
    Gene ID
    50688
    NCBI Accession
    NM_000723
    UniProt
    P54283
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