CACNA1G antibody (Intracellular)

Catalog No. ABIN7043008

Product Details anti-CACNA1G Antibody

Target: CACNA1G (Calcium Channel, Voltage-Dependent, T Type, alpha 1G Subunit (CACNA1G))

Binding Specificity: AA 1-22, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 20,22 amino acid residues identical

Characteristics: Anti-CACNA1G (CaV3.1) Antibody (ABIN7043008, ABIN7043976 and ABIN7043977)) is a highly specific antibody directed against an epitope of the rat CaV3.1 channel. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CACNA1G from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: BLP-CC021

Blocking Peptide: BLP-CC021

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNA1G

Target: CACNA1G (Calcium Channel, Voltage-Dependent, T Type, alpha 1G Subunit (CACNA1G))

Alternative Name: CACNA1G (CACNA1G Products)

Background: Voltage-dependent T-type calcium channel subunit α1G,Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1 T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the CaV channel family.2-3 The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms. CACNA1G (CaV3.1) and CACNA1H (CaV3.2) are widely distributed whereas the expression of CACNA1I (CaV3.3) is restricted to the central nervous system.2CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.

Alternative names: CACNA1G (CaV3.1), Voltage-dependent T-type calcium channel subunit alpha1G (T-Type)

Gene ID: 29717

NCBI Accession: NM_018896

UniProt: O54898