CACNA1H antibody (Intracellular)

Catalog No. ABIN7043010

Product Details anti-CACNA1H Antibody

Target: CACNA1H (Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H))

Binding Specificity: AA 581-595, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to CaV3.2 (CACNA1H) Channel

Specificity: Intracellular loop between domains D1 and D2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse, human - 13,canis - 14,bovine,15 amino acid residues identical

Characteristics: Anti-CaV3.2 (CACNA1H) Antibody (ABIN7043010, ABIN7043980 and ABIN7043981)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV3.2 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of rat CaV3.2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: BLP-CC025

Blocking Peptide: BLP-CC025

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNA1H

Target: CACNA1H (Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H))

Alternative Name: CACNA1H (CACNA1H Products)

Background: Voltage-dependent T-type calcium channel subunit α1H,T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.2 (CACNA1H), CaV3.1 (CACNA1G) and CaV3.3 (CACNA1I).1-3 The CaV3.2 channel is widely expressed in various tissues such as the brain, heart, liver and testis.Involvement of CaV3.2 Ca2+ channels in several pathologies has been described. Overexpression of the CaV3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4 Several point mutations discovered in the CaV3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5 One year old mice, deficient in CaV3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice.6 Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the CaV3.2 Ca2+channels. This might indicate a possible role for CaV3.2 in nociception.7

Alternative names: Cav3.2, alpha1H (T-Type)

Gene ID: 114862

NCBI Accession: NM_001005407

UniProt: Q9EQ60

Pathways: C21-Steroid Hormone Metabolic Process