DLGAP1 antibody (Intracellular)

Catalog No. ABIN7043100

Product Details anti-DLGAP1 Antibody

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Binding Specificity: AA 397-411, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to DLGAP1/GKAP/SAPAP1

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-DLGAP1/GKAP Antibody (ABIN7043100, ABIN7045186 and ABIN7045187) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DLGAP1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235216)

Blocking Peptide: (ABIN7235216)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for DLGAP1

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Alternative Name: DLGAP1 (DLGAP1 Products)

Background: DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1,DLGAP1/GKAP (discs large homolog associated protein 1/guanylate kinase associated protein) is a member of the DLGAP family, scaffold proteins localized to the postsynaptic density. The protein is involved in signaling to and from glutamate receptors, it serves as a key adaptor protein of NMDA receptors, involved in learning and memory and synaptic plasticity in the central nervous system1,2.DLGAP family comprises five conserved proteins, DLGAP1-DLGAP5. DLGAP1-DLGAP4 proteins interact directly with both DLG and SHANK through their multiple domains. DLGAP1-4 proteins are implicated in several neurological disorders including schizophrenia, autism, obsessive compulsive disorder and cerebellar ataxia1.DLGAP1 is highly expressed in the brain, Dlgap1 mRNA is detected in the cortex, hippocampus, olfactory bulb, striatum and thalamus1.

Alternative names: DLGAP1/GKAP, DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1

Gene ID: 65040

NCBI Accession: NM_001003809

UniProt: P97836