F4/80 antibody (Extracellular, N-Term)

Catalog No. ABIN7043117

Product Details anti-F4/80 Antibody

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Binding Specificity: AA 58-72, Extracellular, N-Term

Reactivity: Mouse, Human, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#) raised in guinea pig, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGRE1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-EMR1 (ADGRE1) (extracellular) Antibody (ABIN7043115, ABIN7044269 and ABIN7044270)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: EMR1 (ADGRE1) (EMR1 Products)

Synonyms: EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody

Background: Alternative names: MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Gene ID: 2015

NCBI Accession: NM_001256252

UniProt: Q14246