FFAR2 antibody (Intracellular)

Catalog No. ABIN7043138

The Rabbit Polyclonal anti-FFAR2 antibody has been validated for WB and IHC. It is suitable to detect FFAR2 in samples from Rat.

Quick Overview for FFAR2 antibody (Intracellular) (ABIN7043138)

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FFAR2 Antibody

Binding Specificity: AA 301-314, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize human samples.

Predicted Reactivity: Mouse -12,14 amino acid residues identical

Characteristics: Anti-GPR43/FFAR2 Antibody (ABIN7043138, ABIN7044289 and ABIN7044290) is a highly selective antibody directed against an epitope of the rat Free fatty acid receptor 2. The antibody can be used in western blot analysis. It has been designed to recognize FFAR2 from mouse and rat samples. The antibody is not suited to recognize FFAR2 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RGAEETVEGTKTDR, corresponding to amino acid residues 301-314 of rat FFAR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Cited Application: IFC

Negative Control: (ABIN7235333)

Blocking Peptide: (ABIN7235333)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FFAR2

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Alternative Name: FFAR2

Background: G-protein coupled receptor 43, Free fatty acid receptor 2,Fatty acids have long been recognized for the variety of their effects in the body. Yet, until recently, these actions were thought to be mediated exclusively via actions on cellular metabolism. There are currently three known receptors for free fatty acids: FFA1, FFA2, and FFA3.Free fatty acid receptor 2 (FFA2) is a G-protein coupled receptor containing seven hydrophobic regions, consistent with transmembrane (TM) spanning helices. FFA2 contains cysteine residues in the first and second extracellular loops that are likely to contribute to structure via the formation of intramolecular disulfide bonds. FFA2 mRNA is detected in a number of tissues, but the highest expression is found in immune cells such as neutrophils, monocytes, peripheral blood mononuclear cells, B-lymphocytes, and polymorphonuclear cells.Short chain fatty acids (SCFAs) are fatty acids of less than six carbons and were found to be endogenous agonists of FFA2. The most potent agonist of the receptor is acetate (C2) and propionate (C3) being equipotent followed by butyrate (C4) and then valerate (C5) and formate (C1)1.FFA2 is implicated in the pathogenesis of several diseases. Stimulation of FFA2 by acetate allows resolution of a colitis-related inflammatory response. FFA2 knockout mice show exacerbated or unresolved inflammation in cases of acute and chronic colitis, arthritis, and asthma. In contrast, other studies show that knockout mice exhibit an increased mortality compared to control mice, despite reduced immune cell recruitment, decreased colonic inflammation, and attenuated colonic tissue damage2.

Alternative names: GPR43/FFAR2, G-protein coupled receptor 43, Free fatty acid receptor 2

Gene ID: 292794

NCBI Accession: NM_005306

UniProt: Q76EI6