G Protein-Coupled Receptor 119 antibody (AA 186-200)

Catalog No. ABIN7043147

Product Details anti-G Protein-Coupled Receptor 119 Antibody

Target: G Protein-Coupled Receptor 119 (GPR119)

Binding Specificity: AA 186-200

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G Protein-Coupled Receptor 119 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)

Specificity: 2nd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: human - 13,15 amino acid residues identical,Mouse - 14

Characteristics: Anti-GPR119 Antibody is directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (ABIN7043147, ABIN7044419 and ABIN7044420) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235360)

Blocking Peptide: (ABIN7235360)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for G Protein-Coupled Receptor 119

Target: G Protein-Coupled Receptor 119 (GPR119)

Alternative Name: GPR119 (GPR119 Products)

Background: Glucose-dependent insulinotropic receptor, GPCR2,Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP - suggesting a stimulation of adenylate cyclase, possibly by Gαs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1), this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .

Alternative names: GPR119, Glucose-dependent insulinotropic receptor, GPCR2

Gene ID: 302813

NCBI Accession: NM_178471

UniProt: Q7TQN8

Pathways: Hormone Transport, Peptide Hormone Metabolism