GPR4 antibody (2nd Extracellular Loop)

Catalog No. ABIN7043160

Product Details anti-GPR4 Antibody

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Binding Specificity: 2nd Extracellular Loop, AA 162-177

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Characteristics: Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GPR4 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Alternative Name: GPR4 (GPR4 Products)

Background: Alternative names: GPR4, Acid-sensing G-protein coupled receptor 4, GPR19

Gene ID: 308408

NCBI Accession: NM_005282

UniProt: Q4KLH9