GPR55 antibody (Extracellular)

Catalog No. ABIN7043163

Product Details anti-GPR55 Antibody

Target: GPR55 (G Protein-Coupled Receptor 55 (GPR55))

Binding Specificity: AA 160-174, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR55 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to GPR55

Specificity: 2nd extracellular loop

Cross-Reactivity (Details): The antibody will not recognize the protein from rat or mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human GPR55 (extracellular) Antibody (ABIN7043163, ABIN7044200 and ABIN7044201)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)HGKVEKYMSFHNMSD, corresponding to amino acid residues 160-174 of human GPR55

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:1000

Comment:

Negative Control: BLP-CR061

Blocking Peptide: BLP-CR061

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR55

Target: GPR55 (G Protein-Coupled Receptor 55 (GPR55))

Alternative Name: GPR55 (GPR55 Products)

Background: G-protein coupled receptor 55,GPR55 is an endocannabinoid receptor that belongs to group δ of the rhodopsin-like (class A) receptors. Like all other G-protein coupled receptors, it consists of seven trans-membrane helixes, intracellular C-terminal domain and extracellular N-terminal extension1.The other known cannabinoid receptors, CB1 and CB2 share low homology with GPR55 and have different ligand specificity and different downstream activated cascade. Thus, GPR55 consists on its own a class of cannabinoid receptor2.GPR55 is highly expressed in the striatum and binds a wide range of ligands, among which are endocannabinoids: Anadanine and 2-Arachidonoyl Glycerol, and phytocannabinoids: Tetrahydrocannabinol (THC), abnormal cannabinoid (CBD) and cannabidiol3. Following ligand binding, GPR55 couples to Gα12,13 proteins, which eventually lead to increased intracellular Ca2+ levels2.The endocannabinoid system has a tremendous potential for experimental and clinical research, based on their evolutionary conservation and role in fundamental physiological and pathological processes, including pain modulation, immune function, neuroprotection, cancer, cardiovascular diseases, fertility and appetite4. Clinical studies using cannabinoid receptor agonists and antagonists have suggested their possible use in modulating acute and/or chronic diseases such as hypertension, glaucoma, emesis, anxiety, depression, gastrointestinal and hepatic disorders, ulcerative colitis and neuropathic and inflammatory pain5.

Alternative names: G-protein coupled receptor 55

Gene ID: 9290

NCBI Accession: NM_005683

UniProt: Q9Y2T6