GPR65 antibody (Extracellular)

Catalog No. ABIN7043167

Product Details anti-GPR65 Antibody

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Binding Specificity: AA 72-84, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR65 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Live Cell Imaging (LCI), Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to GPR65 (TDAG8)

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rat - 11,Mouse - 12,13 amino acid residues identical

Characteristics: Anti-GPR65 (TDAG8) (extracellular) Antibody (ABIN7043167, ABIN7044427 and ABIN7044428) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize GPR65 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: (ABIN7235434)

Blocking Peptide: (ABIN7235434)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR65

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Alternative Name: GPR65 (GPR65 Products)

Background: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor,GPR65, also called TDAG8 (T-cell death associated gene 8 protein), is a pH -sensing G-protein coupled receptor (GPCR) activated by acidic extracellular pH through the protonation of several histidine residues in the receptor's sequence. GPR65 is involved in cancer cell metastasis and proliferation, immune cell function, inflammation, and blood vessel formation1.Human GPR65 gene has been mapped to a location that is associated with T cell lymphoma and leukemia abnormalities.GPR65 is primarily expressed in immune cells and leukocyte-rich tissues such as circulating peripheral leukocytes, spleen, thymus, and tonsils. Expression is also detected pain relevant loci such as the dorsal root ganglia neurons and particularly small diameter neurons responsible for nociception1,2.GPR65 has the ability to affect tumor development and growth. Overexpression of GPR65 in Lewis lung carcinoma cells increases tumor growth in mice and may facilitate resistance to acidosis-mediated cell death in vitro through protein kinase A (PKA) and ERK related pathways1.Knockdown of GPR65 in NCI-H460 human non-small cell lung cancer cells decreases cell survival in acidic condition. Another study shows that activation of GPR65 by acidosis can promote evasion of cell apoptosis under glutamine starvation and its overexpression has been reported to transform immortalized mammary epithelial cells1.

Alternative names: GPR65 (TDAG8), G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor

Gene ID: 8477

NCBI Accession: NM_003608

UniProt: Q8IYL9

Pathways: cAMP Metabolic Process