GJC1 antibody (Intracellular)

Catalog No. ABIN7043207

Product Details anti-GJC1 Antibody

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Binding Specificity: AA 153-166, Intracellular

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-Connexin-45 (GJC1) Antibody is directed against an epitope of rat Connexin-45. Anti-Connexin-45 (GJC1) Antibody (ABIN7043207, ABIN7044085 and ABIN7044086)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx45 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJC1

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Alternative Name: Connexin-45 (GJC1) (GJC1 Products)

Background: Alternative names: Connexin-45 (GJC1), CX45, Gap junction gamma1 protein, Gap junction alpha7 protein, GJA7

Gene ID: 266706

NCBI Accession: NM_005497

UniProt: A4GG66

Pathways: Cell-Cell Junction Organization