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Gephyrin antibody (Intracellular)

GPHN Reactivity: Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043214
  • Target See all Gephyrin (GPHN) Antibodies
    Gephyrin (GPHN)
    Binding Specificity
    • 7
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 183-195, Intracellular
    Reactivity
    • 49
    • 33
    • 26
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    Rat
    Host
    • 42
    • 11
    • 2
    • 1
    Rabbit
    Clonality
    • 39
    • 17
    Polyclonal
    Conjugate
    • 34
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Gephyrin antibody is un-conjugated
    Application
    • 37
    • 16
    • 14
    • 14
    • 6
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    A Rabbit Polyclonal Antibody to Gephyrin
    Specificity
    Intracellular
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Mouse,human - identical
    Characteristics
    Anti-Gephyrin Antibody is directed against an epitope of rat Gephyrin. Anti-Gephyrin Antibody (ABIN7043214, ABIN7044520 and ABIN7044521) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPHN from rat, mouse, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

    Isotype
    IgG
    Top Product
    Discover our top product GPHN Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: (ABIN7235588)

    Blocking Peptide: (ABIN7235588)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Gephyrin (GPHN)
    Alternative Name
    GPHN (GPHN Products)
    Background
    GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase,Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

    Alternative names: Gephyrin, GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase
    Gene ID
    64845
    NCBI Accession
    NM_020806
    UniProt
    Q03555
    Pathways
    Synaptic Membrane, Skeletal Muscle Fiber Development
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