GRM8 antibody (Extracellular)

Catalog No. ABIN7043256

Product Details anti-GRM8 Antibody

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Binding Specificity: AA 365-377, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-mGluR8 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR8. Anti-mGluR8 (extracellular) Antibody (ABIN7043256 and ABIN7044363)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize mGluR8 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-GC028

Blocking Peptide: BLP-GC028

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRM8

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Alternative Name: GRM8 (GRM8 Products)

Background: Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H,Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2.The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels.Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.

Alternative names: mGluR8, Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H

Gene ID: 60590

NCBI Accession: NM_000845

UniProt: P70579

Pathways: cAMP Metabolic Process, Synaptic Membrane