HRH4 antibody (Extracellular)

Catalog No. ABIN7043272

Product Details anti-HRH4 Antibody

Target: HRH4 (Histamine Receptor H4 (HRH4))

Binding Specificity: AA 75-87, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Human Histamine H4 Receptor

Specificity: 1st extracellular loop

Predicted Reactivity: Won't recognize rat,mouse H4 Histamine Receptor

Characteristics: Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody is directed against an epitope located at the 1st extracellular loop of the human histamine H4 receptor. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (ABIN7043272, ABIN7044510 and ABIN7044511)) can be used in western blot and indirect flow cytometry applications, and recognizes H4R from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IFC

Negative Control: BLP-HR004

Blocking Peptide: BLP-HR004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HRH4

Target: HRH4 (Histamine Receptor H4 (HRH4))

Alternative Name: HRH4 (HRH4 Products)

Background: H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1- H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Histamine H4 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.1,2Histamine H4 receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2,3These studies indicate that histamine H4 receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2,3

Alternative names: Histamine H4 Receptor (HRH4), H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

Gene ID: 59340

NCBI Accession: NM_001143828

UniProt: Q9H3N8