HOMER2 antibody (Intracellular)

Catalog No. ABIN7043274

Product Details anti-HOMER2 Antibody

Target: HOMER2 (Homer Homolog 2 (HOMER2))

Binding Specificity: AA 302-316, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOMER2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Homer2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Homer2 Antibody (ABIN7043274, ABIN7045170 and ABIN7045171)) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer2 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PZ027

Blocking Peptide: BLP-PZ027

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HOMER2

Target: HOMER2 (Homer Homolog 2 (HOMER2))

Alternative Name: HOMER2 (HOMER2 Products)

Background: Homer protein homolog 2, Cupidin, Vesl-2,Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1. The Homer family includes three members, Homer1b/c, Homer2a/b, and Homer3. Homer2 protein, also called Vesl-2, is a scaffolding protein that is important for the proper organization, localization and signaling of excitatory postsynaptic receptors. Homer2 protein associates with filamentous actin (F-actin) and directly binds to drebrin, an actin-binding and remodeling protein in dendritic spines. The protein also binds to activated Cdc42, a member of the Rho small GTPase family2. The protein interacts with mGluR1, mGluR5, PI3 Kinase enhancer (PIKE), inositol 1,4,5-trisphosphate receptors (IP3), SHANK and other proteins3,4.Deletion of the Homer2 gene in mice demonstrates several abnormalities in glutamatergic signaling and in the nucleus accumbens such as reduced basal extracellular glutamate content and reduced function and total protein expression of both mGluR1 and the cystine-glutamate exchanger4.

Alternative names: Homer2, Homer protein homolog 2, Cupidin, Vesl-2

Gene ID: 29547

NCBI Accession: NM_004839

UniProt: O88801

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling