HVCN1 antibody (Intracellular)

Catalog No. ABIN7043276

Product Details anti-HVCN1 Antibody

Target: HVCN1 (Hydrogen Voltage-Gated Channel 1 (HVCN1))

Binding Specificity: AA 32-44, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HVCN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to HVCN1 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-HVCN1 Antibody is directed against an intracellular epitope of mouse HVCN1 channel. Anti-HVCN1 Antibody (ABIN7043276, ABIN7044501 and ABIN7044502)) can be used in western blot and indirect flow cytometry applications. It has been designed to recognize HVCN1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GDDYHTWNVNYKK, corresponding to amino acid residues 32-44 of mouse HVCN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: BLP-HC001

Blocking Peptide: BLP-HC001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HVCN1

Target: HVCN1 (Hydrogen Voltage-Gated Channel 1 (HVCN1))

Alternative Name: HVCN1 (HVCN1 Products)

Background: Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, VSOP,Currents measured from voltage-gated proton channels were detected1 long before the channel (HVCN1, also known as HV1 and VSOP) was cloned2,3. HVCN1 has four membrane spanning domains and intracellular N- and C-termini. Interestingly, unlike its voltage-gated ion channel counterparts, HVCN1 has no pore4. Also, functional HVCN1 channels are formed by dimers where each monomer has its own conducting pore, each with its own voltage sensor (voltage sensing occurs similarly to other voltage-gated ion channels)5-8. The fundamental role of HVCN1 is to pump out protons, thereby increasing the intracellular pH . The channel is exclusively selective for H+ and opens upon membrane depolarization, although its open state hugely depends on the pH on both sides of the membrane4. Its role is best described in leukocytes where phosphorylation via PKC on a Thr residue potentiates the activity of the channel and increases its open state, thereby increasing the H+ current across the membrane, in this manner mediating optimal NADPH-oxidase (whose optimal activity is at pH 7.5) required for the production of reactive oxygen species (ROS) necessary for phagocytosis to occur4.Apart from leukocytes, HVCN1 is also expressed in basophils where its activation mediates histamine release4,9. In B cells, it maintains optimal signaling, such that ROS production is maintained high10. HVCN1 was found to regulate human spermatozoa activation. Finally, in the airway mucosa, where it regulates pH , channel gating there, is mostly mediated by differences in pH across the membrane as opposed to the membrane potential4.

Alternative names: HVCN1, Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, VSOP

Gene ID: 74096

NCBI Accession: NM_032369

UniProt: Q3U2S8

Pathways: Proton Transport