KIDINS220 antibody (Intracellular)

Catalog No. ABIN7043293

Product Details anti-KIDINS220 Antibody

Target: KIDINS220 (Kinase D-Interacting Substrate, 220kDa (KIDINS220))

Binding Specificity: AA 1335-1348, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIDINS220 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Kidins220

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Kidins220 Antibody (ABIN7043293, ABIN7045160 and ABIN7045161)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kidins220 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PRHSNLSWQSQTRR, corresponding to amino acid residues 1335-1348 of rat Kidins220

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-PZ017

Blocking Peptide: BLP-PZ017

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KIDINS220

Target: KIDINS220 (Kinase D-Interacting Substrate, 220kDa (KIDINS220))

Alternative Name: KIDINS220 (KIDINS220 Products)

Background: Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS,The Kidins220 (Kinase D-interacting substrate of 220 kDa) or ARMS (Ankyrin repeat-rich membrane-spanning) protein was originally identified as a substrate for protein kinase D (PKD) in neural cells. Kidins220 is a member of the Kidins220/ARMS and PifA (KAP) family of P-loop nucleotide phosphatases (NTPases).Kidins220 is an integral membrane protein, containing four transmembrane segments in the central part of the molecule, and N- and C-terminal tails both exposed to the cytoplasm. Kidins220 possesses numerous domains that mediate protein-protein interactions. The N-terminus of Kidins220 contains eleven ankyrin repeats which form a concave binding surface that is accessible to molecular partners. Kidins220 contains Walker A and Walker B (involved in the binding of nucleotides) motifs in its juxta-membrane regions flanking the N- and C-termini. The C-terminus of Kidins220 includes several protein-protein interaction domains that are involved in inter- and intra-molecular interactions. The last four amino acids of Kidins220 constitute a PDZ-binding motif that binds the PDZ domain of α-syntrophin, thereby forming a complex that is associated with the EphA4 receptor at the neuromuscular junction1.Neurotrophin signaling is responsible for a wide variety of neuronal functions and is dependent upon two different transmembrane receptor types, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor that belongs to the TNF receptor family. Kidins220 interacts with TrkA, TrkB, TrkC receptors, and with p75NTR. Kidinis220 is rapidly tyrosine phosphorylated after binding of neurotrophins to Trk receptors and provides a docking site for the CrkL-C3G complex, resulting in Rap1-dependent sustained ERK activation2.Kidins220 also associates with NMDA receptors. Upon overstimulation of the NMDA receptor, activity-dependent Ca2+ influx through NMDA receptors leads to a decrease in Kidins220 levels owing to transcriptional downregulation and protein cleavage by calpain1.

Alternative names: Kidins220, Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS

Gene ID: 116478

NCBI Accession: NM_020738

UniProt: Q9EQG6