LRRC4B antibody (Extracellular)

Catalog No. ABIN7043300

Product Details anti-LRRC4B Antibody

Target: LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))

Binding Specificity: AA 498-511, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRC4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to NGL-3/LRRC4B

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, human - 13 out of 14 amino acid residues identical

Characteristics: Anti-NGL-3/LRRC4B (extracellular) Antibody (ABIN7043300, ABIN7044736 and ABIN7044737)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR163

Blocking Peptide: BLP-NR163

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LRRC4B

Target: LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))

Alternative Name: LRRC4B (LRRC4B Products)

Background: Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4,NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.Mice lacking NGL-3 (Ngl3-/-) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development6.

Alternative names: NGL-3/LRRC4B, Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4

Gene ID: 272381

NCBI Accession: NM_001080457

UniProt: P0C192

Pathways: Synaptic Membrane