Nerve Growth Factor antibody (Intracellular)

Catalog No. ABIN7043341

Product Details anti-Nerve Growth Factor Antibody

Target: Nerve Growth Factor (NGF)

Binding Specificity: Intracellular

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity (Details): The antibody does not react with BDNF, NT-3, and NT-4/5.

Characteristics: Anti-NGF Antibody is directed against mouse NGF. Anti-NGF Antibody (#AN-240) can be used in western blot and immunohistochemical applications. It has been designed to recognize NGF from mouse, rat and human samples. The antibody also recognizes proNGF. \nRelated products for neutralizing NGF activity: \nMouse NGF/proNGF Neutralizing Antibody (#) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Highly purified 2.5 S mouse NGF

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.3 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 5 % sucrose (no preservative).

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF (NGF Products)

Background: Alternative names: NGF, Nerve growth factor

Gene ID: 18049

NCBI Accession: NM_002506

UniProt: P01139

Pathways: Regulation of Cell Size