O3FAR1 antibody (Extracellular)

Catalog No. ABIN7043397

Product Details anti-O3FAR1 Antibody

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Binding Specificity: AA 22-36, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This O3FAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-GPR120/FFAR4 (extracellular) Antibody (ABIN7043397, ABIN7044281 and ABIN7044282)) is a highly specific antibody directed against an epitope of the mouse Free fatty acid receptor 4. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize FFAR4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-FR014

Blocking Peptide: BLP-FR014

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for O3FAR1

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Alternative Name: FFAR4 (O3FAR1 Products)

Background: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1,Free fatty acid receptor 4 (FFAR4, GPR120), is a member of the rhodopsin family of 7-transmembrane domain G-protein coupled receptors (GPCRs). GPR120/FFAR4 is activated by long chain fatty acids. The protein is a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM). GPR120 is associated to insulin sensitizing, anti-inflammatory, and fat metabolism. Evidence shows that GPR120 may be involved in the development of obesity in mice and humans1-4. GPR120 regulates various physiological processes, including gut hormone secretion, islet function, osteoclastogenesis, anti-inflammation, and adipogenesis2-4.GPR120 binds to omega-3 fatty acids and stabilizes the metabolic homeostasis through a cascade of physiological activities. GPR120 exerts its physiological effects through one of two pathways that involves either Gαq or β-arrestin2,3.GPR120 has been shown to be ubiquitously expressed and is primarily detected in the intestine, adipocytes, and pro-inflammatory macrophages1,2.

Alternative names: GPR120/FFAR4 (extracellular), GPR120, FFA4 Receptor , Free fatty acid receptor 4, Omega-3 fatty acid receptor 1, G-protein coupled receptor 120, G-protein coupled receptor GT01, O3far1

Gene ID: 107221

NCBI Accession: NM_181745

UniProt: Q7TMA4

Pathways: Hormone Transport