OPRL1 antibody (Intracellular) (Atto 594)

Catalog No. ABIN7043400

Product Details anti-OPRL1 Antibody (Atto 594)

Target: OPRL1 (Opiate Receptor-Like 1 (OPRL1))

Binding Specificity: AA 337-352, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPRL1 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,16 amino acid residues identical

Characteristics: Anti-Nociceptin Receptor (OPRL1) Antibody (ABIN7043399, ABIN7044893 and ABIN7044894) is a highly specific antibody directed against an epitope the rat protein. The antibody can be used in western blot analysis, and has been designed to recognize the nociceptin receptor from mouse, rat and human samples. \nAnti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody (ABIN7043400) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7236167)

Blocking Peptide: (ABIN7236167)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for OPRL1

Target: OPRL1 (Opiate Receptor-Like 1 (OPRL1))

Alternative Name: OPRL1 (OPRL1 Products)

Background: κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor,Nociceptin/orphanin FQ (N/OFQ), a 17 amino acid peptide, is the endogenous agonist of the nociceptin/orphanin FQ peptide (NOP) receptor (previously termed ORL1 receptor) which belongs to the class A (Rhodopsin-like) γ subfamily of G protein-coupled receptors (GPCRs). Like all members the receptor has a common seven-transmembrane helical structure and is coupled predominantly to heterotrimeric Gi/Go proteins. N/OFQ receptor shows a high degree of structural homology with the μ-, δ- and κ-opioid receptors1. The intracellular events triggered by the binding of N/OFQ with the NOP receptor include inhibition of adenylyl cyclase, activation of phospholipase C (PLC) and of K+ channels, inhibition of Ca2+ channels, and activation of mitogen-activated protein-kinases (MAPKs). In neural tissues, the main effect is the inhibition of the release of neurotransmitters (acetylcholine, catecholamines, GABA, glutamate, 5-hydroxytryptamine, tachykinines)2.NOP receptor mRNA is detected in high levels in the cortex, anterior olfactory nucleus, lateral septum, hypothalamus, hippocampus, amygdala, central grey, pontine nuclei, interpeduncular nucleus, substantia nigra, raphe complex, locus coeruleus and spinal cord3.Studies with N/OFQ, NOP-selective agonists or antagonists, and receptor- or peptide-deficient mice have shown that the NOP system has important roles in the control of central and peripheral functions including pain, anxiety depression, hyperphagia and obesity, addiction, Parkinson's disease, cognition, food intake, learning and memory, locomotion, cough and micturition reflexes, cardiovascular homeostasis, intestinal motility and immune responses4.

Alternative names: Nociceptin Receptor (OPRL1), Kappa-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor

Gene ID: 29256

NCBI Accession: NM_182647

UniProt: P35370