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ORAI1 antibody (Extracellular)

ORAI1 Reactivity: Human WB, IF, LCI, IC, IF (cc) Host: Mouse Monoclonal 3F11-D10-B9 unconjugated
Catalog No. ABIN7044554
  • Target See all ORAI1 Antibodies
    ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
    Binding Specificity
    • 8
    • 7
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Extracellular
    Reactivity
    • 52
    • 24
    • 8
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 49
    • 5
    • 1
    Mouse
    Clonality
    • 50
    • 5
    Monoclonal
    Conjugate
    • 29
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ORAI1 antibody is un-conjugated
    Application
    • 41
    • 30
    • 14
    • 8
    • 8
    • 4
    • 4
    • 4
    • 3
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC), Immunofluorescence (Cultured Cells) (IF (cc))
    Purpose
    A Mouse Monoclonal Antibody to Human Orai1 Channel
    Specificity
    Extracellular
    Cross-Reactivity (Details)
    Recognizes only human Orai1.
    Predicted Reactivity
    Orai1 from human samples only
    Characteristics
    Mouse Anti-Human Orai1 (extracellular) Antibody (ABIN7043409 and ABIN7044554) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: Synthetic mapping to an extracellular sequence of human Orai1

    Clone
    3F11-D10-B9
    Isotype
    IgM
    Top Product
    Discover our top product ORAI1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: NA

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
    Alternative Name
    ORAI1 (ORAI1 Products)
    Background
    TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1,Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified, Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

    Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
    Gene ID
    84876
    NCBI Accession
    NM_032790
    UniProt
    Q96D31
    Pathways
    TCR Signaling, BCR Signaling
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