P2RY2 antibody (Extracellular)

Catalog No. ABIN7043424

Product Details anti-P2RY2 Antibody

Target: P2RY2 (Purinergic Receptor P2Y, G-Protein Coupled, 2 (P2RY2))

Binding Specificity: AA 11-24, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to P2Y2 Receptor

Specificity: Extracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat, human - 13,mouse - 14,15 amino acid residues identical

Characteristics: Anti-P2Y2 Receptor (extracellular) Antibody (ABIN7043424, ABIN7045138 and ABIN7045139)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, live cell imaging, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: BLP-PR102

Blocking Peptide: BLP-PR102

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RY2

Target: P2RY2 (Purinergic Receptor P2Y, G-Protein Coupled, 2 (P2RY2))

Alternative Name: P2RY2 (P2RY2 Products)

Background: P2Y purinoceptor 2, P2RY2, P2U1,P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation1.The mammalian P2Y family currently includes eight functional receptors, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y142-4.The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway5. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6

Alternative names: P2Y2 Receptor, P2Y purinoceptor 2, P2RY2, P2U1

Gene ID: 29597

NCBI Accession: NM_176072

UniProt: P41232

Pathways: Cellular Response to Molecule of Bacterial Origin, Smooth Muscle Cell Migration