Pannexin 2 antibody (Extracellular)

Catalog No. ABIN7043427

Product Details anti-Pannexin 2 Antibody

Target: Pannexin 2 (PANX2)

Binding Specificity: AA 76-90, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pannexin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Pannexin 2

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse,rat - 14,15 amino acid residues identical

Characteristics: Anti-Pannexin 2 (extracellular) Antibody (ABIN7043427, ABIN7044096 and ABIN7044097)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize PANX2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EEPIY(S)YTPHNFTRD, corresponding to amino acid residues 76-90 of human PANX2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CC232

Blocking Peptide: BLP-CC232

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Pannexin 2

Target: Pannexin 2 (PANX2)

Alternative Name: PANX2 (PANX2 Products)

Background: PANX2, PX2, hPANX2,Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9.

Alternative names: Pannexin 2, PANX2, PX2, hPANX2

Gene ID: 56666

NCBI Accession: NM_052839

UniProt: Q96RD6