Pannexin 3 antibody (AA 156-170)

Catalog No. ABIN7043428

Product Details anti-Pannexin 3 Antibody

Target: Pannexin 3 (PANX3)

Binding Specificity: AA 156-170

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pannexin 3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Pannexin 3

Specificity: Intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, mouse - 13,15 amino acid residues

Characteristics: Anti-Pannexin 3 Antibody is directed against an epitope of human Pannexin 3. Anti-Pannexin 3 Antibody (ABIN7043428, ABIN7044098 and ABIN7044099)) can be used in western blot analysis. It was designed to recognize PANX3 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RLVQHMLKIRQKSSD, corresponding to amino acid residues 156-170 of human PANX3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CC233

Blocking Peptide: BLP-CC233

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Pannexin 3

Target: Pannexin 3 (PANX3)

Alternative Name: PANX3 (PANX3 Products)

Background: PANX3, PX3,Pannexins are a class of membrane channels similar to the invertebrate Connexin family of gap channel proteins. There are currently three known types of pannexin channels and the exact role of these channels is yet to be fully discerned1.A recent study suggests that despite similarity to the connexin family these channels are not gap-junctions or "hemichannels" as described in some publications but fully operational channels. These conclusions were derived because pannexins, unlike gap-junctions, are found to be expressed and functional in single membranes facing the extracellular space and not as intercellular membranes in appositional cells.Pannexins are comprised of four transmembrane segments, two extracellular loops and both the amino and carboxy termini are facing the cytosol. Pannexins also have four conserved extracellular loop cysteines resulting in the possibility of two intra-pannexin disulfide bonds. Pannexin 3 has a glycosylation site at N254 and N71. Pannexin membrane channels are formed by 6-8 monomers and while pannexins 3's exact oligomeric number has not been determined yet it is expected to be 6 with a greater similarity to Pannexin 1 than 22.There are two known subtypes of Panx3 in mice with different molecular weight and distribution. The first, weighing 43 kDa is mostly expressed in cartilage skin tissues and also in low levels in heart ventricle tissue. The second, weighing 70 kDa, and possibly functioning as a dimer is detected in lung, liver, thymus and spleen tissues3.Since full understanding of pannexin 3 structure, expression and role in physiological and pathological tissues is yet to be obtained there is a definite need for further research.

Alternative names: Pannexin 3, PANX3, PX3

Gene ID: 116337

NCBI Accession: NM_052959

UniProt: Q96QZ0