KCNK4 antibody (Intracellular)

Catalog No. ABIN7043454

Product Details anti-KCNK4 Antibody

Target: KCNK4 (Potassium Channel, Subfamily K, Member 4 (KCNK4))

Binding Specificity: AA 343-359, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KCNK4 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,dog - identical

Characteristics: Anti-KCNK4 (TRAAK) Antibody (ABIN7043454, ABIN7045004 and ABIN7045005)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-PC108

Blocking Peptide: BLP-PC108

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK4

Target: KCNK4 (Potassium Channel, Subfamily K, Member 4 (KCNK4))

Alternative Name: KCNK4 (KCNK4 Products)

Background: Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1,KCNK4 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or K2P4.1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leaky" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini.K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK4 can be integrated to a K2P subfamily that includes K2P2.1 (TREK1) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid, high intracellular pH and mechanical stretch.KCNK4 expression is largely confined to the brain in mouse samples while in humans it is also expressed in placenta and to a lesser degree in kidney, small intestine and prostate.

Alternative names: KCNK4 (TRAAK), Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1

Gene ID: 50801

NCBI Accession: NM_033311

UniProt: Q9NYG8