KCNT2 antibody (Intracellular)

Catalog No. ABIN7043458

Product Details anti-KCNT2 Antibody

Target: KCNT2 (Potassium Channel, Subfamily T, Member 2 (KCNT2))

Binding Specificity: AA 991-1004, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KCNT2 (Slick)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical, human - 10,Mouse - 13

Characteristics: Anti-KCNT2 (Slick) Antibody is directed against an epitope located at the intracellular C-terminal domain of rat KCNT2. Anti-KCNT2 (Slick) Antibody (ABIN7043458, ABIN7045029 and ABIN7045030)) can be used in western blot and immunocytochemistry applications. It has been designed to recognize KCNT2 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KDVKDPGHHRSIHR, corresponding to amino acid residues 991-1004 of rat Slick 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-PC126

Blocking Peptide: BLP-PC126

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNT2

Target: KCNT2 (Potassium Channel, Subfamily T, Member 2 (KCNT2))

Alternative Name: KCNT2 (KCNT2 Products)

Background: KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2,KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) was originally cloned and named so due to its high similarity to the Slo genes1. Shortly after its discovery, KCa4.2 (Slick, Slo2.1, KCNT2), its sister channel, was also cloned2. Although KCa4.2 (like KCa4.1) is functionally a Na+-activated K+ channel (KNa), it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels. Both channels are activated by high intracellular concentrations of Na+.Like Slack, Slick contains six transmembrane spanning domains, a P-region between transmembrane regions 5 and 6 and intracellular N- and C-termini. However, the N-terminal domain of Slick is significantly shorter than that of Slack. In addition, contrary to Slack, Slick is regulated by ATP as it has an ATP binding site in its C-terminal domain2. ATP binding reduces the activity of the channel and mutations of this site abolish the inhibitory effect2. Both channels are regulated by intracellular Cl- ions, but Slick displays higher sensitivity3. Also, the overall electrical characteristics of Slick channels are different from those of Slack, Slick is rapidly activated in response to depolarization, and also has a basal level of activity in the absence of Na+. Both channels are highly expressed in the brain with overlapping expression. Slick is found in the midbrain, brainstem, and hippocampus and throughout the neocortex. This KNa channel is also detected in the auditory neurons in the brainstem. Detection of Slick was also found in the heart, although at much lower levels2.Many different functions have been attributed to KNa channels including action potential repolarization, slow after-hyperpolarization, burst firing and adaptation after repetitive firing3. These channels also contribute to the response of neurons to hypoxia.

Alternative names: KCNT2 (Slick), KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2

Gene ID: 304827

NCBI Accession: NM_198503

UniProt: Q6UVM4