KCNJ10 antibody (Intracellular)

Catalog No. ABIN7043468

Product Details anti-KCNJ10 Antibody

Target: KCNJ10 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10))

Binding Specificity: AA 356-375, Intracellular

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This KCNJ10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Guinea Pig Polyclonal Antibody to Kir4.1 (KCNJ10) Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Kir4.1 potassium channel from human, rat, and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir4.1 (KCNJ10) Antibody (ABIN7043469, ABIN7044943 and ABIN7044944)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KLEESLREQAEKEGSALSVR, corresponding to amino acid residues 356-375 of rat Kir4.1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:800

Comment:

Negative Control: BLP-PC035

Blocking Peptide: BLP-PC035

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNJ10

Target: KCNJ10 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10))

Alternative Name: KCNJ10 (KCNJ10 Products)

Background: ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10,Kir4.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney1,2.It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the K+ released during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential3.

Alternative names: Kir4.1, ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel Inwardly rectifying subfamily J member 10

Gene ID: 29718

NCBI Accession: NM_002241

UniProt: P49655

Pathways: Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity