KCNS3 antibody (Extracellular)

Catalog No. ABIN7043498

Product Details anti-KCNS3 Antibody

Target: KCNS3 (Potassium Voltage-Gated Channel, Delayed-Rectifier, Subfamily S, Member 3 (KCNS3))

Binding Specificity: AA 209-223, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KCNS3 (KV9.3) Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-KCNS3 (Kv9.3) (extracellular) Antibody is directed against an extracellular epitope of the rat KV9.3 channel. Anti-KCNS3 (KV9.3) (extracellular) Antibody (ABIN7043498 and ABIN7045036) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV9.3 from human mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EFQNEDGEVDDPVLE, corresponding to amino acid residues 209-223 of rat KCNS3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236433)

Blocking Peptide: (ABIN7236433)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNS3

Target: KCNS3 (Potassium Voltage-Gated Channel, Delayed-Rectifier, Subfamily S, Member 3 (KCNS3))

Alternative Name: KCNS3 (KCNS3 Products)

Background: Potassium voltage-gated channel subfamily S member 3,K+ channels are transmembrane proteins expressed in many excitable and non-excitable cells. Functional entities are formed by the tetrameric assembly of α subunits which could be done in a homomeric or heteromeric fashion. In addition, the association of β subunits is also required for the proper function of K+ channels. Various splice variants are also expressed, thereby complicating the picture1,2.K+ channels belonging to the KV9 subfamily resemble the delayed-rectifier class of K+ channel α subunits. These channels include six transmembrane domains, an ion selective pore, a leucine zipper and positively charged amino acids in S4 the voltage sensor domain. Interestingly, both KV9.1 and KV9.3 channels are electrically silent delayed rectifying K+ channels. However, they are responsible for modifying the activity of other K+ channels such as that of KV2.1, yielding currents different from those of KV2.1 on its own1.KV9.1 is mainly expressed in the brain, human lens epithelial cells, kidney, prostate and testis. That of KV9.3 is more generalized and ubiquitous1.

Alternative names: KCNS3 (KV9.3), Potassium voltage-gated channel subfamily S member 3

Gene ID: 83588

NCBI Accession: NM_002252

UniProt: O88759