KCNA5 antibody (Extracellular)

Catalog No. ABIN7043529

Product Details anti-KCNA5 Antibody

Target: KCNA5 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 5 (KCNA5))

Binding Specificity: AA 268-279, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Rat

Predicted Reactivity: mouse - 9,Rat - 12,13 amino acid residues identical, human - 11

Characteristics: Anti-KV1.5 (KCNA5) (extracellular) Antibody (ABIN7043529, ABIN7045046 and ABIN7045047)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry. This antibody recognizes an extracellular epitope and thus is ideally suited to detect KV1.5 in living cells. It has been designed to recognize KV1.5 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DERELLRHPPVP(K), corresponding to amino acid residues 268-279 of rat KV1.5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|ICC

Negative Control: BLP-PC150

Blocking Peptide: BLP-PC150

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNA5

Target: KCNA5 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 5 (KCNA5))

Alternative Name: KCNA5 (KCNA5 Products)

Background: Potassium voltage-gated channel subfamily A member 5,KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from the rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss-of-function mutation in the gene encoding the channel was found in atrial fibrillation patients, stressing its role as a cardiac action potential regulator.3The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the "classical" non-selective potassium channel blockers.2

Alternative names: KV1.5 (KCNA5), Potassium voltage-gated channel subfamily A member 5

Gene ID: 25470

NCBI Accession: NM_002234

UniProt: P19024