KCNH6 antibody (Intracellular)

Catalog No. ABIN7043549

Product Details anti-KCNH6 Antibody

Target: KCNH6 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 6 (KCNH6))

Binding Specificity: AA 185-198, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KV11.2 (ERG-2) Channel

Specificity: Intracellular, N-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,chicken - identical, mouse - 13,14 amino acid residues identical

Characteristics: Anti-KCNH6 (erg2) Antibody (ABIN7043549 and ABIN7045012) directed against a highly conserved epitope of the human KV11.2 channel. The antibody can be used to detect KV11.2 in rat samples using both western blotting and immunohistochemistry applications.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human KV11.2 (erg2)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236581)

Blocking Peptide: (ABIN7236581)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNH6

Target: KCNH6 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 6 (KCNH6))

Alternative Name: KCNH6 (KCNH6 Products)

Background: KV11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2,KV11.2 (erg2) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins KV11.1 (erg1) and KV11.3 (erg3) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the KV11 subfamily members can form functional heteromultimers within the subfamily.KV11.2 produces currents characterized by strong inward rectification with slow activation and very rapid inactivation kinetics, which closely resemble those produced by the much studied channel KV11.1.The expression of KV11.2 seems to be limited to the brain and the pituitary gland. The same is true for the KV11.3 protein, while KV11.1 is more widely expressed. From a pharmacological point of view, KV11.2 channels can be blocked by well characterized organic blockers such as the anti-arrhythmic drug E-4031. In addition, the peptide toxins Ergtoxin-1 and BeKm-1 block KV11.2 with different potencies.

Alternative names: KCNH6 (erg2), KV11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2

Gene ID: 81033

NCBI Accession: NM_030779

UniProt: Q9H252