Prostaglandin D2 Receptor 2 (PTGDR2) (AA 270-283), (Extracellular) antibody

Catalog No. ABIN7043564

Product Details

Target: Prostaglandin D2 Receptor 2 (PTGDR2)

Binding Specificity: AA 270-283, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Prostanoid DP2 Receptor (CRTH2, GPR44)

Specificity: 3rd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize DP2 receptor from human samples.

Predicted Reactivity: Mouse - identical Will not recognize DP2 receptor from human samples

Characteristics: Anti-CRTH2/GPR44 (extracellular) Antibody (ABIN7043564, ABIN7045118 and ABIN7045119)) is a highly selective antibody directed against an epitope of the rat Prostanoid DP2 receptor. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize DP2 receptor from mouse and rat samples. It is unlikely to recognize the receptor from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-PR062

Blocking Peptide: BLP-PR062

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: Prostaglandin D2 Receptor 2 (PTGDR2)

Alternative Name: PTGDR2 (PTGDR2 Products)

Background: Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294,Prostanoids are potent oxygenated lipid molecules that contribute to a wide variety of physiological responses and pathological processes. There are currently five primary bioactive types of prostanoids: PGD2, PGE2, PGF2R, PGI2, and thromboxane A2 (TXA2).All prostanoid receptors belong to the G-protein coupled receptor superfamily. Unlike other receptors of this family the Prostaglandin D2 receptor (also known as CRTH2, GPR44) is structurally distinct and belongs to the family of leukocyte chemoattractant receptors.The DP2 receptor is expressed in Th2 cells, basophils, and eosinophils, and it mediates the chemotaxis of these cells in response to PGD2. The ability of PGD2 to promote eosinophil accumulation in the airways is mimicked by selective DP2 agonists but not DP1 agonists.Much effort has been directed toward developing DP2 agonists and antagonists. Indomethacin and ramatroban, which act, respectively, as an agonist and an antagonist, were the first nonprostanoid compounds to be identified as DP2 ligands. These two compounds have frequently used as leads for identifying new selective compounds1.

Alternative names: CRTH2/GPR44, Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294

Gene ID: 309212

NCBI Accession: NM_004778

UniProt: Q6XKD3

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling