P2RY4 antibody (Intracellular)

Catalog No. ABIN7043589

Product Details anti-P2RY4 Antibody

Target: P2RY4 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 4 (P2RY4))

Binding Specificity: AA 337-350, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC), Immunoprecipitation (IP)

Purpose: A Rabbit Polyclonal Antibody to P2Y4 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 13,14 amino acid residues identical

Characteristics: Anti-P2Y4 Receptor Antibody (ABIN7043589, ABIN7045078 and ABIN7045079)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY4 in rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:300

Comment:

Cited Application: IHC

Negative Control: BLP-PR006

Blocking Peptide: BLP-PR006

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RY4

Target: P2RY4 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 4 (P2RY4))

Alternative Name: P2RY4 (P2RY4 Products)

Background: P2Y purinoceptor 4, P2RY4, Uridine nucleotide receptor,The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4The P2Y4-receptor is highly expressed in the placenta and with lower levels in the lung and vascular smooth muscle.5The human P2Y4 receptor is highly selective to UTP while rat P2Y4 receptor is equally activated by both UTP and ATP and also by other triphosphate nucleotides such as Ap4A.5Recently it has been shown that P2Y4 receptor is involved in differentiation and cell death of human neuroblastoma SH-SY5Y cells.6

Alternative names: P2Y4 Receptor, P2Y purinoceptor 4, P2RY4, Uridine nucleotide receptor

Gene ID: 63843

NCBI Accession: NM_002565

UniProt: O35811