P2RY6 antibody (Intracellular)

Catalog No. ABIN7043590

Product Details anti-P2RY6 Antibody

Target: P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))

Binding Specificity: AA 311-328, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to P2Y6 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 18 amino acid residues identical, human - 15,Mouse - 17

Characteristics: Anti-P2Y6 Receptor Antibody (ABIN7043590, ABIN7045086 and ABIN7045087)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY6 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|IFC

Negative Control: BLP-PR011

Blocking Peptide: BLP-PR011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RY6

Target: P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))

Alternative Name: P2RY6 (P2RY6 Products)

Background: P2Y purinoceptor 6, P2RY6,The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily.1,3P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels.1,2P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries.4

Alternative names: P2Y6 Receptor, P2Y purinoceptor 6, P2RY6

Gene ID: 117264

NCBI Accession: NM_001277204

UniProt: Q63371

Pathways: Smooth Muscle Cell Migration