RTN4R antibody (Extracellular)

Catalog No. ABIN7043601

Product Details anti-RTN4R Antibody

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Binding Specificity: AA 366-381, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RTN4R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Nogo Receptor

Specificity: Extracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 15,16 amino acid residues identical

Characteristics: Anti-Nogo Receptor (extracellular) Antibody is directed against an extracellular epitope of the human Nogo receptor. Anti-Nogo Receptor (extracellular) Antibody (ABIN7043601, ABIN7044757 and ABIN7044758)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-NT008

Blocking Peptide: BLP-NT008

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for RTN4R

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Alternative Name: RTN4R (RTN4R Products)

Background: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R,The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3 Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein. Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7

Alternative names: Nogo Receptor, NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Gene ID: 65078

NCBI Accession: NM_023004

UniProt: Q9BZR6

Pathways: Neurotrophin Signaling Pathway