SHANK1 antibody (Intracellular)

Catalog No. ABIN7043626

Product Details anti-SHANK1 Antibody

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Binding Specificity: AA 614-628, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHANK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Shank1

Specificity: Intracellular, between the SH3 and PDZ domains.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize all known rat isoforms of Shank1 with the exception of isoform 2 (see Q9WV48-2).

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Characteristics: Anti-Shank1 Antibody is directed against an epitope of rat Shank1. Anti-Shank1 Antibody (ABIN7043626, ABIN7045152 and ABIN7045153)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Shank1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSQEGRQESRSDKAK, corresponding to amino acid residues 614-628 of rat Shank1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-PZ011

Blocking Peptide: BLP-PZ011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SHANK1

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Alternative Name: SHANK1 (SHANK1 Products)

Background: SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon,Shank family proteins (shank1, shank2 and shank3) are neuronal scaffold proteins that organize a cytoskeleton-associated signaling complex at the postsynaptic density (PSD) of nearly all excitatory glutamatergic synapses in the mammalian brain1.The Shank family, including Shank1, has five conserved protein domains: 1. ankyrin repeat domain (ANK), 2. Src homology 3 (SH3) domain, 3. PSD-95/Discs large/ZO-1 (PDZ) domain, 4. proline-rich region containing homer and cortactin-binding sites (Pro), 5. sterile α motif (SAM) domain2. All these domains can act as sites for specific protein-protein interactions, and the presence of these multiple domains within a single polypeptide is consistent with a function as a scaffolding protein. Shanks interact directly or indirectly with all major types of glutamate receptors: NMDA receptors, AMPA receptors, and mGluRs via different domains. Shank proteins also interact with several actin regulatory proteins and L-type Ca2+ (CaV1.3) channels in medium spiny neurons of the striatum3.Shank1 mRNA, being synthesized in dendritic spine, can locally control the spine morphology. The Shank1 gene is located on chromosome 19q13.33 and contains 23 exons and two alternative promoters leading to two distinct isoforms4. Shank1 is highly expressed in the cortex and hippocampus5.Recent studies indicate that Shank1 is a causative gene for idiopathic autism spectrum disorders (ASD)1. In addition it was found that Shank1 has a role in working memory deficits in schizophrenia6. Shank1 knock-out mice display increased anxiety and decreased vocal communication7.

Alternative names: Shank1, SH3 and multiple ankyrin repeat domains protein 1, SPANK-1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon

Gene ID: 78957

NCBI Accession: NM_016148

UniProt: Q9WV48

Pathways: Synaptic Membrane, Maintenance of Protein Location