Nav1.8 antibody (Intracellular)

Catalog No. ABIN7043653

Product Details anti-Nav1.8 Antibody

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Binding Specificity: AA 1943-1956, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nav1.8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Rat

Predicted Reactivity: 15 amino acid residues identical,Mouse - 14, human - 7

Characteristics: Anti-NaV1.8 (SCN10A) Antibody (ABIN7043653, ABIN7045239 and ABIN7045240)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.8 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: BLP-SC016

Blocking Peptide: BLP-SC016

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Nav1.8

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Alternative Name: SCN10A (SCN10A Products)

Background: PN3, SNS, Sodium channel protein type 10 subunit alpha,Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 Expression of the α subunit isoform is developmentally and tissue specific.Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6

Alternative names: NaV1.8 (SCN10A), PN3, SNS, Sodium channel protein type 10 subunit alpha

Gene ID: 29571

NCBI Accession: NM_006514

UniProt: Q63554