Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

NALCN antibody (Extracellular)

NALCN Reactivity: Rat WB, IF, IHC, IC, LCI Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043658
  • Target See all NALCN Antibodies
    NALCN (Sodium Leak Channel, Non-Selective (NALCN))
    Binding Specificity
    • 12
    • 5
    • 1
    AA 902-914, Extracellular
    Reactivity
    • 14
    • 5
    • 2
    • 1
    Rat
    Host
    • 18
    • 5
    Rabbit
    Clonality
    • 18
    • 5
    Polyclonal
    Conjugate
    • 10
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NALCN antibody is un-conjugated
    Application
    • 18
    • 11
    • 11
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC), Live Cell Imaging (LCI)
    Purpose
    A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    Specificity
    Extracellular, S1-S2 domain III.
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Mouse,human - identical
    Characteristics
    Anti-NALCN/VGCNL1 (extracellular) Antibody (ABIN7043658 and ABIN7045249) is a highly specific antibody directed against an epitope of rat Sodium leak channel non-selective protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NALCN from mouse, rat, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN

    Isotype
    IgG
    Top Product
    Discover our top product NALCN Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: (ABIN7236886)

    Blocking Peptide: (ABIN7236886)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    NALCN (Sodium Leak Channel, Non-Selective (NALCN))
    Alternative Name
    NALCN (NALCN Products)
    Background
    Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel,The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) been found responsible for the resting Na+ conductance1.Nalcn is a member of the NaV and CaV channel family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2.Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die within 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1.The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3.In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.

    Alternative names: NALCN/VGCNL1, Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel
    Gene ID
    266760
    NCBI Accession
    NM_052867
    UniProt
    Q6Q760
You are here:
Support