SLC1A2 antibody (2nd Extracellular Loop) (Atto 633)

Catalog No. ABIN7043661

Product Details anti-SLC1A2 Antibody (Atto 633)

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Binding Specificity: 2nd Extracellular Loop

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A2 antibody is conjugated to Atto 633

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-EAAT2 (GLT-1) (extracellular) Antibody (ABIN7043660, ABIN7044354 and ABIN7044355)) is a highly specific antibody directed against an epitope of the rat Excitatory amino acid transporter 2. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. It has been designed to recognize EAAT2 from human, rat, and mouse samples. \nAnti-EAAT2 (GLT-1) (extracellular)-ATTO Fluor-633 Antibody (#ABIN7043661) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-EAAT2 (GLT-1) (extracellular)-ATTO Fluor-633 Antibody has been tested in immunohistochemical staining and is specially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KQLGPGKKNDEVS, corresponding to amino acid residues 151-163 of rat EAAT2

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC1A2

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Alternative Name: EAAT2 (GLT-1) (SLC1A2 Products)

Background: Alternative names: EAAT2 (GLT-1), Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2, SLC1A2

Gene ID: 29482

NCBI Accession: NM_004171

UniProt: P31596

Pathways: Dicarboxylic Acid Transport