SLC1A7 antibody (Extracellular)

Catalog No. ABIN7043664

Product Details anti-SLC1A7 Antibody

Target: SLC1A7 (Solute Carrier Family 1 (Glutamate Transporter), Member 7 (SLC1A7))

Binding Specificity: AA 122-134, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A7 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (SLC1A7)

Specificity: 2nd extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-SLC1A7 (extracellular) Antibody (ABIN7043664, ABIN7044359 and ABIN7044360) is a highly selective antibody directed against an epitope of mouse Excitatory amino acid transporter 5. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize EAAT5 from mouse, human, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KETTEQSGKPVMS, corresponding to amino acid residues 122-134 of mouse EAAT5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236902)

Blocking Peptide: (ABIN7236902)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC1A7

Target: SLC1A7 (Solute Carrier Family 1 (Glutamate Transporter), Member 7 (SLC1A7))

Alternative Name: SLC1A7 (SLC1A7 Products)

Background: Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter,Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells throughout the body with glutamate for metabolic purposes.SLC1A7 (EAAT5) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters1. EAAT5 is expressed mainly in photoreceptors and bipolar cells in the retina and is essential for the maintenance of normal excitatory synaptic transmission as it determines the time course of glutamate receptor activation. EAAT5 consists of 3 identical subunits that are functionally coupled to the sodium (Na+), potassium (K+)-ATPase as part of the same macromolecular complex. The transporter operates via an "alternate access mechanism" whereby the translocation of one molecule of glutamate into the cell is coupled with the inward cotransport with 3Na+ and 1H+ ions. In turn, the return of the empty carrier to the extracellular side of the plasma membrane is coupled to the export of 1K+ ion. In addition, EAAT5, may function as glutamate-gated chloride (Cl-) channel, generating an anion current that is not stoichiometrically coupled to the uptake of glutamate2.EAAT5 has been linked to the development of hippocampal sclerosis. Hippocampal sclerosis of aging (HS-Aging) is a causative factor in a large proportion of elderly dementia cases3.

Alternative names: SLC1A7, Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter

Gene ID: 242607

NCBI Accession: NM_006671

UniProt: Q8JZR4

Pathways: Dicarboxylic Acid Transport