Solute Carrier Family 17 (Acidic Sugar Transporter), Member 5 (SLC17A5) (AA 479-492), (Intracellular) antibody

Catalog No. ABIN7043679

Product Details

Target: Solute Carrier Family 17 (Acidic Sugar Transporter), Member 5 (SLC17A5)

Binding Specificity: AA 479-492, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to SLC17A5 (Sialin) Transporter

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Rat - 13, human - 12

Characteristics: Anti-SLC17A5 (Sialin) Antibody (ABIN7043679, ABIN7045310 and ABIN7045311)) is a highly specific antibody directed against an epitope of the mouse Sialin transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Sialin from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KGEVQSWALSDHHG, corresponding to amino acid residues 479-492 of mouse SLC17A5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-ST015

Blocking Peptide: BLP-ST015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: Solute Carrier Family 17 (Acidic Sugar Transporter), Member 5 (SLC17A5)

Alternative Name: SLC17A5 (SLC17A5 Products)

Background: Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter,Sialin, encoded by the SLC17A5 gene, is a member of the SLC17 solute carrier family, a group of structurally related polytopic membrane proteins, part of the major facilitator superfamily of transporters. Sialin is a sialic acid transporter located in the lysosomal membrane. In the lysosome, sialic acid residues are sequentially removed from the carbohydrate chain by sialidases and then transported out of the lysosome by the transporter protein sialin1,2. Sialin structure consists of 12 transmembrane helices (TMs). TM4 contains three residues (Arg-168, Glu-175, and His-183) that are highly conserved in the SLC17 family, suggesting that they may participate directly in substrate binding and translocation. In addition, TM4 contains a GXXXG helix packing motif that is important for the expression of sialin. SLC17 family includes several more GXXXG-like motifs located within TMs including GXXXG/S in TM2, G/AXXXG in TM5, and G/S/AXXXGXXXG/T in TM13.Proteins included in the SLC17 family are implicated with inherited neurological and metabolic diseases. Mutations in the SLC17A5 gene cause sialic acid storage diseases including infantile sialic acid storage disease and Salla disease. These conditions are autosomal recessive neurodegenerative disorders characterized by excessive accumulation of sialic acid in the lysosome1,2.

Alternative names: SLC17A5 (Sialin), Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter

Gene ID: 235504

NCBI Accession: NM_001382629

UniProt: Q8BN82