SLC6A4 antibody (Extracellular)

Catalog No. ABIN7043745

Product Details anti-SLC6A4 Antibody

Target: SLC6A4 (Solute Carrier Family 6 (Neurotransmitter Transporter, serotonin), Member 4 (SLC6A4))

Binding Specificity: AA 388-400, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT)

Specificity: 4th extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Serotonin Transporter (SERT) (extracellular) Antibody (ABIN7043745, ABIN7044608 and ABIN7044609)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, indirect flow cytometry, and live cell imaging applications. It has been designed to recognize SERT from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat SERT

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|ICC

Negative Control: BLP-MT004

Blocking Peptide: BLP-MT004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC6A4

Target: SLC6A4 (Solute Carrier Family 6 (Neurotransmitter Transporter, serotonin), Member 4 (SLC6A4))

Alternative Name: SLC6A4 (SLC6A4 Products)

Background: Sodium-dependent serotonin transporter, 5-HT transporter, 5-HTT, 5-HTTLPR, SLC6A4,Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3. The intensity of monoaminergic signaling is determined by the availability of the monoamine, which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin (5-HT) and noradrenaline respectively, and recycling them back for release3-5.DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. Like its counterparts, SERT's intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization6.SERTs are evidently expressed in serotonergic neurons, and are also expressed in various peripheral tissues including specialized cells of the gut, placenta, lung, blood lymphocytes and platelets6.SERT knockout mice are viable and as a consequence exhibit increased extracellular levels of 5-HT and significantly decreased levels of brain tissue 5-HT due to deficient recycling and re-accumulation of serotonin by SERT7. Phenotypes displayed by SERT knockout mice include stress sensitivity, obesity and various behavioral changes. 8

Alternative names: Serotonin Transporter (SERT), Sodium-dependent serotonin transporter, 5-HT transporter, 5-HTT, 5-HTTLPR, SLC6A4

Gene ID: 25553

NCBI Accession: NM_001045

UniProt: P31652